TITLE: What goes out must come in: coupling of synaptic exo- and endocytosis
AUTHOR: Jurgen Klingauf, Ph.D.
TIME: 12:00:00 PM DATE: Monday,June 8, 2015
PLACE: Porter Neuroscience Research Center
Live NIH Videocast (archived after seminar)
Reseaerch Group Leader
Max Planck Institute for Biophysical Chemistry
The focus of our research is the study of synaptic transmission, with the emphasis on presynaptic mechanisms. At the synapse, neurotransmitter is rapidly released from small vesicles which are triggered to fuse with the plasma membrane by the entry of Ca2+ ions.
Phone: +49 251 83-51001
Address: Institute of Medical Physics and Biophysics
The maintenance of synaptic transmission requires that these vesicles be retrieved by a reverse process, i.e. endocytosis. How is this endocytic activity and subsequent formation of fusion-competent vesicles coupled to exocytosis? To delineate the mechanisms by which synaptic vesicles can be retrieved we employ high-resolution imaging techniques, like two-photon laser scanning and total internal reflection microscopy, electrophysiology, as well as biochemical approaches. By transfection of neurons in primary cell culture or the usage of knock-out models we can target or modulate specific proteins thought to be pivotal in synaptic vesicle endocytosis. Currently, we are mainly studying synapses of rodent hippocampus, down to the level of single fluorescently labeled vesicles in cultured or freshly isolated synaptic boutons. By making use of fluorescent styryl dyes with different kinetic properties we found that in central nervous synapses at least two kinetically distinct modes of endocytosis co-exist. We are now trying to characterize the respective molecular events underlying those different mechanisms using genetically encoded fluorescent probes.
About the NIH Neuroscience Seminars
The NIH Neuroscience Seminar Series features lectures and discussions with leading neuroscientists. Sponsored by NINDS, NIMH, NIA, NIDCD, NIDA, NICHD,NEI,NIAAA,NIDCR, NHGRI and NCCIH, this year’s series offers seminars on aspects of molecular, cellular, developmental and cognitive neuroscience as well as neuroscience related topics in disease, pain and genetics. Seminars are held on the NIH campus on Mondays at noon in the Porter Neuroscience Research Center, Room 620/630, Building 35.